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simplified method for the preparation of detergent-free lipid rafts test

A sensitive S-Trap-based approach to the analysis of T ...- simplified method for the preparation of detergent-free lipid rafts test ,The analysis of T cell lipid raft proteome is challenging due to the highly dynamic nature of rafts and the hydrophobic character of raft-resident proteins. We explored an innovative strategy for bottom-up lipid raftomics based on suspension-trapping (S-Trap) sample preparation. Mouse T cells were prepared from splenocytes by negative immunoselection, and rafts were isolated …Native low density lipoprotein promotes lipid raft ...Jan 14, 2016·Macdonald JL and Pike LJ: A simplified method for the preparation of detergent-free lipid rafts. J Lipid Res. 46:1061–1067. 2005. View Article : …



Isolation and Analysis of Lipid Rafts in Cell ... - Springer

Lipid rafts are dynamic structures made up of proteins and lipids ... L. J. (2005) A simplified method for the preparation of detergent-free lipid rafts. J. Lipid Res. 46, 1061–1067 ... Goda, K., Andrasfalvy, M., et al. (2004) Cholesterol sensitivity of detergent resistance: a rapid flow cytometric test for detecting constitutive or induced ...

Lipid rafts and regulation of the cytoskeleton during T ...

Aug 16, 2005·2003 Lipid raft distribution of CD4 depends on its palmitoylation and association with Lck, and evidence for CD4-induced lipid raft aggregation as an additional mechanism to enhance CD3 signaling. J. Immunol. 170, 913–921. Crossref, PubMed, ISI, Google Scholar

Lipid Raft Microdomains: A Gateway for Compartmentalized ...

Preparation of Detergent Insoluble Fractions and Lipid Rafts. Lipid rafts were prepared after lysing the cells in lysis buffer con-taining 0.5% Triton X-100 as described previously (13). Raft and soluble fractions were then analyzed by immunoblotting. In some experiments (see Fig. 3 A), detergent-insoluble fractions were ex-

The Unconventional Role of Acid Sphingomyelinase in ...

Aug 20, 2011·RESEARCH DESIGN AND METHODS. ... Factorial ANOVA with post hoc Tukey test (GraphPad Prism5; GraphPad Software, Inc., San Diego, CA) was used for comparing the data obtained from independent samples. Significance was ... A simplified method for the preparation of detergent-free lipid rafts. J Lipid Res 2005; 46:1061–1067 [Google ...

Pathogens | Free Full-Text | The Lipid Raft Proteome …

Aug 24, 2017·Lipid rafts were isolated using a detergent-free method of MacDonald and Pike . 8 × 10 8 cells of 29-13 PF T. brucei were pelleted by centrifugation at 1000× g. Cells were washed twice with PBS supplemented …

Interaction of ganglioside GD3 with an EGF receptor ...

Nov 19, 2013·To test how the EGFR level was influenced in GD3S −/ ... Preparation of Detergent-Free Lipid Raft Fractions. ... Macdonald JL, Pike LJ. A simplified method for the preparation of detergent-free lipid rafts. J Lipid Res. …

EGF induces coalescence of different lipid rafts

Aug 01, 2008·Detergent-free lipid raft fractions were purified and examined for the presence of EGFR, GPI-GFP and MAP kinase by western blotting, and GM1 by a dot-blot assay using CTB-HRP. EGF receptors, GPI-GFP and GM1 are all present in the lipid raft fraction, whereas MAP kinase is clearly absent.

Methods for the Study of Dopamine Receptors Within Lipid ...

The isolation of lipid rafts can be performed using either detergent-based or detergent-free methods (Yu et al., 2013), with the latter generating a greater fraction of inner leaflet membrane ...

Identification of GPCR Localization in Detergent Resistant ...

Preparation of Lipid Raft-rich Membranes from HEK293 Cells ... We report here a simplified method for the purification of detergent-free lipid rafts …

Native low density lipoprotein promotes lipid raft ...

Jan 14, 2016·Macdonald JL and Pike LJ: A simplified method for the preparation of detergent-free lipid rafts. J Lipid Res. 46:1061–1067. 2005. View Article : …

A simplified method for the preparation of detergent-free ...

Feb 16, 2005·A simplified method for the preparation of detergent-free lipid rafts. Jennifer L Macdonald Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 South Euclid Avenue, St. Louis, MO 63110, USA.

Mechanisms Responsible for the Compositional Heterogeneity ...

A simplified method for the preparation of detergent-free lipid rafts. J. Lipid Res. 2005; 46 : 1061-1067 View in Article

Role for Rab10 in Methamphetamine-Induced Behavior

Aug 20, 2015·Striata were rapidly dissected on an ice-chilled petri dish and frozen on dry ice until used to prepare raft fractions or for subcellular fractionation. Rafts were separated using a modified detergent-free method [11]. Briefly, striata from 2 rats were combined, homogenized with a Polytron and passed 20 times through a 20G needle.

CiteSeerX — lipid rafts

Abstract. A simplified method for the preparation of detergent-free. Keyphrases. lipid raft simplified method

Carbonate step gradient and OptiPrep detergent free ...

Lipid-rafts/caveolae in VSMCs stimulated with/without Ang II for 5, 15 or 30 min were isolated by protein fractionation using the detergent-free sucrose gradient centrifugation method as we ...

A method for detergent-free isolation of membrane proteins ...

Jun 02, 2016·Jamshad, M. et al. Structural analysis of a nanoparticle containing a lipid bilayer used for detergent-free extraction of membrane proteins. Nano Res. 8 …

Polyunsaturated fatty acid supplements ... - ScienceDirect

Jan 01, 2012·A simplified method for the preparation of detergent-free lipid rafts J. Lipid Res. , 46 ( 2005 ) , pp. 1061 - 1067 Article Download PDF View Record in Scopus Google Scholar

A simplified method for the preparation of detergent-free ...

May 01, 2005·Simplified method for the preparation of detergent-free lipid rafts All procedures were carried out on ice. Four D150 plates of cells were washed and scraped into base buffer (20 mM Tris-HCl, pH 7.8, 250 mM sucrose) to which had been added 1 mM CaCl 2 …

A simplified method for the preparation of detergent-free ...

Lipid rafts are small plasma membrane domains that contain high levels of cholesterol and sphingolipids. Traditional methods for the biochemical isolation of lipid rafts involve the extraction of cells with nonionic detergents followed by the separation of a low-density, detergent-resistant membrane fraction on density gradients.

Role of Membrane Microdomains in Serotonin Receptor Functions

Nov 15, 2017·Lipid rafts, detergent-resistant membranes, and raft targeting signals. ... Macdonald JL. A simplified method for the preparation of detergent-free lipid rafts. J Lipid Res. 2005;46:1061–7. ... of the anti-immobility action of antidepressants by a selective 5-HT7 receptor antagonist in the forced swimming test in mice. Eur J Pharmacol. 2007 ...

Interaction of ganglioside GD3 with an EGF receptor ... - PNAS

Nov 19, 2013·To test the association of GD3 with integrin and EGFR, ... Preparation of Detergent-Free Lipid Raft Fractions. The procedure for preparing detergent-free fractions was modified from MacDonald and Pike ... (2005) A simplified method for the preparation of detergent-free lipid rafts.

Pathogens | Free Full-Text | The Lipid Raft Proteome …

Aug 24, 2017·Lipid rafts were isolated using a detergent-free method of MacDonald and Pike . 8 × 10 8 cells of 29-13 PF T. brucei were pelleted by centrifugation at 1000× g. Cells were washed twice with PBS supplemented …

Aggregation of lipid rafts activates c-met and c-Src in ...

May 30, 2018·Macdonald JL, Pike LJ. A simplified method for the preparation of detergent-free lipid rafts. J Lipid Res. 2005;46:1061–7. Article PubMed CAS Google Scholar Song KS, Li S, Okamoto T, Quilliam LA, Sargiacomo M, Lisanti MP.

Macrophage ABCA1 reduces MyD88-dependent Toll-like ...

A simplified method for the preparation of detergent-free lipid rafts. J. Lipid Res. 2005; 46 : 1061-1067 View in Article